Journal: PLoS Pathogens

Article Title: Cellular N-myristoyltransferases play a crucial picornavirus genus-specific role in viral assembly, virion maturation, and infectivity

doi: 10.1371/journal.ppat.1007203

Figure Lengend Snippet: (A) Work flow for detection of Alk-12 labeled viral proteins with click reagents. HeLa cells (DDD85646- or DMSO-treated) are infected with CVB3 at MOI of 10. The myristic acid analogue Alk-12 is added 4 h p.i. and incubation continued for 3 h; during this time Alk-12 becomes transferred to viral substrate proteins by metabolic incorporation. Cells are lysed and Alk-12-bearing proteins are ligated to the fluorescent reporter 5-TAMRA-azide by click chemistry, separated by SDS-PAGE and visualized by in-gel fluorescence. (B) DDD85646 at 5 μM blocks incorporation of Alk-12 into CVB3 VP0. The gel subjected to in-gel fluorescence recording was subsequently stained with InstantBlue to visualize total proteins for verifying equal loading.

Article Snippet: Click chemistry grade Na-ascorbate, Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), Cu 2 SO 4 , and the fluorescent capturing reagents Cy5.5-Alkyne and 5-TAMRA-Azide were from Jena Biosciences.

Techniques: Labeling, Infection, Incubation, SDS Page, Fluorescence, Staining

Journal: Molecular Systems Biology

Article Title: Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics

doi: 10.1038/s44320-024-00058-1

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Carboxytetramethylrhodamine (TAMRA)-Azide-Polyethylene Glycol (PEG)-Desthiobiotin , BroadPharm , BP-22475.

Techniques: Transfection, Luciferase, Recombinant, Software